Ivermectin/Abamectin, ELISA, 96-test

The Ivermectin/Abamectin Plate Kit is a competitive ELISA for the quantitative analysis of Ivermectin/Abamectin in honey products. The Ivermectin/Abamectin plate kit is a competitive enzyme-labeled immunoassay. Ivermectin/Abamectin is extracted from a sample by blending or shaking with extraction solution. The sample extract and calibrators are pipetted into the test wells followed by Ivermectin/Abamectin-HRP conjugate solution. An Ivermectin/Abamectin antibody solution is then added into the test wells to initiate the reaction. During the 30 minute incubation period, Ivermectin/Abamectin from the sample and Ivermectin/Abamectin HRP conjugate compete for binding to Ivermectin/Abamectin antibody. The Avermectin antibodies are bound by a second antibody immobilized on the microtiter plate wells. Following a 30 minute incubation, the contents of the wells are removed and the wells are washed to remove any unbound Ivermectin/Abamectin, and Ivermectin/Abamectin HRP conjugate. A clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 30 minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Ivermectin/Abamectin concentration of the samples is derived by interpolation using a standard curve constructed with each run.

PN 5142B

Made to Order - Minimum Order Quantity 5 Kits

Format: 96-Well Plate ELISA