ABRAXIS® Acrylamide-ES, ELISA, 96-test

The Acrylamide-ES ELISA is an immunoassay for the detection of Acrylamide in foods. This test is suitable for the quantitative and/or qualitative detection of Acrylamide in food samples such as potato/corn chips, french fries, cereals, and breads. For food sample applications, contact Gold Standard Diagnostics for the appropriate technical bulletin and/or matrix validation guidelines. An alternative clean-up procedure (SPE Array Vacuum) technical bulletin is available upon request. Samples requiring action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Acrylamide by specific antibodies. Acrylamide, when present in a sample, and an Acrylamide-enzyme conjugate compete for the binding sites of rabbit anti-Acrylamide antibodies in solution. The Acrylamide antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the microtiter plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Acrylamide present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

PN 515690

Made to Order - Minimum Order Quantity 5 Kits

Format: 96-Well Plate ELISA