ABRAXIS® Atrazine, Magnetic Particle ELISA, 100-test

Magnetic particle kit for detection and quantitation of atrazine and related triazines in water (groundwater, surface water, well water). For soil, crop, and food use contact the company for application bulletins and/or specific matrix validation guidelines. The Atrazine Kit applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of atrazine and related triazines. The sample to be tested is added, along with an enzyme conjugate, to a disposable test tube, followed by paramagnetic particles attached with antibodies specific to triazines. At this point a competitive reaction occurs between the triazine which may be be in the sample and the enzyme labeled atrazine for the antibody binding sites on the magnetic particles. The reaction is allowed to continue for fifteen (15) minutes. At the end of the incubation period, a magnetic field is applied to hold in the test tube the para-magnetic particles (with atrazine and labeled atrazine bound to the antibodies on the particles, in proportion to their original concentration), and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution. The presence of atrazine is detected by adding the Color Solution, which contains the enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5'-tetramethyl-benzidine). The enzyme-labeled atrazine bound to the atrazine antibody catalyzes the conversion of the substrate/ chromogen mixture to a colored product. After an incubation period, the reaction is stopped and stabilized by the addition of a diluted acid (Stopping Solution). Since the labeled atrazine (conjugate) was in competition with the unlabeled atrazine (sample) for the antibody sites, the color developed is inversely proportional to the concentration of atrazine in the sample.

PN 500001

Format: Magnetic Particle Test