ABRAXIS® Dicamba, ELISA, 96-tests

The ABRAXIS® Dicamba ELISA Plate Test Kit is an immunoassay for the quantitative and sensitive screening of Dicamba in contaminated water, durum wheat or soil samples. The test is a direct competitive ELISA based on the recognition of Dicamba by specific antibodies. The standards, control, and samples are added to microtiter wells coated with polyclonal anti-Dicamba antibodies, followed by Dicamba enzyme conjugate. A competitive reaction occurs between the Dicamba (which may be present in the sample) and the enzyme labelled Dicamba for the binding sites of the anti-Dicamba antibodies immobilized on the microtiter plate. The reaction is allowed to incubate for 60 minutes, followed by a washing step. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Dicamba present in the sample. The color reaction is stopped after a specified time and is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run. Samples requiring regulatory action should be confirmed by HPLC, LC-MS/MS, or other conventional methods.

PN 500050

Format: 96-test