ABRAXIS® DDE/DDT, ELISA, 96-test

The DDE/DDT ELISA is an immunoassay for the quantitative and sensitive screening of DDE/DDT. This test is suitable for the quantitative and/or qualitative screening of DDE/DDT in water samples (groundwater, surface water, and well water). For soil and other sample matrices, please contact Abraxis technical services for application bulletins and/or specific matrix validation guidelines. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of DDE/DDT by specific antibodies. DDE/DDT, when present in a sample, and a DDE/DDT-HRP analogue compete for the binding sites of the anti-DDE/DDT antibodies in solution. The DDE/DDT antibodies are then bound by a second antibody (anti-mouse) immobilized on the wells of the microtiter plate. After a washing step and the addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of DDE/DDT present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

PN 540041

Made to Order - Minimum Order Quantity 5 Kits

Format: 96-Well Plate ELISA