ABRAXIS® Salinomycin/Narasin, ELISA, 96-test

The Salinomycin/Narasin ELISA is an immunoassay for the quantitative and sensitive screening of Salinomycin and Narasin. This test is suitable for the quantitative and/or qualitative screening of Salinomycin and Narasin in animal feed and contaminated products (please refer to the appropriate technical bulletins for extraction/dilution procedures for additional matrices). Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Salinomycin and Narasin by specific antibodies. Salinomycin and Narasin, when present in a sample, and a Salinomycin-HRP analogue compete for the binding sites of rabbit anti-Salinomycin antibodies in solution. The Salinomycin antibodies are then bound by a second antibody (donkey anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Salinomycin and/or Narasin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

PN 515795

Format: 96-Well Plate ELISA