ABRAXIS® PCBs Higher Chlorinated, ELISA, 96-test

ABRAXIS® PCBs Higher Chlorinated, ELISA, 96-test

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The PCB ELISA is an immunoassay for the quantitative and sensitive screening of Polychlorinated Biphenyls (PCBs). This test is suitable for the quantitative and/or qualitative screening of PCBs in water and soil samples (please refer to the appropriate technical bulletins for additional matrices procedures). Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Polychlorinated Biphenyls by specific antibodies. The PCBs, when present in a sample, and a PCB-HRP analogue compete for the binding sites of anti-PCB antibodies in solution. The PCB antibodies are then bound by a second antibody immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of PCBs present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

PN 530041

Made to Order - Minimum Order Quantity 5 Kits

Format: 96-Well Plate ELISA