ABRAXIS® Microcystins/Nodularins (ADDA) SAES, ELISA, 96-test

The Microcystins-ADDA SAES ELISA is an enhanced sensitivity immunoassay for the quantitative and sensitive congener-independent detection of Microcystins and Nodularins in water samples. This test is suitable for the quantitative and/or qualitative detection of Microcystins and Nodularins in water samples [please refer to the appropriate technical bulletins for sample collection, handling, and treatment of drinking (treated and untreated) and recreational water samples]. If necessary, positive samples can be confirmed by HPLC, protein phosphatase assay, or other conventional methods. The test is an indirect competitive ELISA for the congener-independent detection of Microcystins and Nodularins. It is based on the recognition of Microcystins, Nodularins, and their congeners by specific biotinylated antibodies. Toxin, when present in a sample, and a Microcystins-protein analogue immobilized on the plate compete for the binding sites of the biotinylated anti-Microcystins/Nodularins antibodies in solution. The plate is then washed and a streptavidin-HRP is added. After a second washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Learn more about our Algal Toxin Proficiency Testing Program

PN 520011SAES

Format: 96-Well Plate ELISA