D/E Neutralizing Broth (w/o Tween)

D/E Neutralizing Broth is commonly used for environmental sampling in areas where antimicrobial compounds may be used. The composition of D/E Neutralizing Broth effectively neutralizes a broad spectrum of antimicrobial compounds, allowing organisms to grow in the presence of otherwise bacteriostatic agents. The inclusion of lecithin serves to neutralize quaternary ammonium compounds. Sodium thiosulfate neutralizes iodine and chlorine. Sodium Thioglycollate neutralizes mercurial while sodium bisulfite neutralizes aldehydes. Polysorbate 80, if added, neutralizes phenolic compounds.

Casein peptone and yeast extract provide a nitrogen source and essential vitamins for growth. Dextrose serves as the main energy source. The inclusion of Bromocresol Purple allows for the detection of glucose utilization, as the resulting pH shift will cause the medium to turn from purple to yellow.

PN DCM3001; 500g

PN DCM3010; 10kg